Utilization
of Bagasse Cellulose for Ethanol Production through Simultaneous
Saccharification and
Fermentation
by Xylanase.
Abstract
Bagasse is a solid
residue from sugar cane process, which is not many use it for some
product which have
more added value. Bagasse, which is a lignosellulosic material, be able to be
use for alternative
energy resources like
bioethanol or biogas. With renewable energy resources a crisis of energy in
Republic of Indonesia
could be solved,
especially in oil and gas. This research has done the conversion of bagasse to
bioethanol with xylanase
enzyme. The result
show that bagasse contains of 52,7% cellulose, 20% hemicelluloses, and 24,2%
lignin. Xylanase
enzyme and
Saccharomyces cerevisiae was used to hydrolyse and fermentation in SSF process.
Variation in this
research use pH (4,
4,5, and 5), for increasing ethanol
quantity, SSF process was done by added chloride acid (HCl)
with concentration
0.5% and 1% (v/v) and also pre-treatment with white rot fungi such as Lentinus
edodes (L.edodes)
as long 4 weeks. The
SSF process was done with 24, 48, 72, and 96 hour’s incubation time for
fermentation. Variation
of pH 4, 4,5, and 5
can produce ethanol with concentrations
2,357 g/L, 2,451 g/L, 2,709 g/L. The added chloride acid
(HCl) with
concentration 0.5% and 1% (v/v) and L. edodes can increase ethanol yield, The
highest ethanol
concentration with
added chloride acid (HCl) concentration 0.5% and 1% consecutively is 2,967 g/L, 3,249 g/L. The
highest ethanol
concentration with pre-treatment by L. edodes is 3,202 g/L.
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